Anti-Cyclin A Antibody from BioLegend

January 27, 2022

Division of Cancer Sciences
University of Manchester
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Anti-Cyclin A

Catalog Number:

644001

Clone Number:

E23.1

This is antibody works well for detecting Cyclin A in U2OS cells via immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

U2OS cells

Primary Incubation

1:500, 4 C, overnight

Blocking Agent

1% BSA in TBST

Secondary Incubation

1:10000, anti-mouse HRP conjugated antibody, 1 h, RT

Tertiary Incubation

N/A

Detection

Chemiluminescence, ECL western blotting substrate

Results Summary

This antibody works well for detecting the Cyclin A protein (~50 kD) in U2OS cell lysates via immunoblotting.

DOI or PMID #

Additional Notes

Related Categories

Image Gallery

Summary

The Good

Good antibody for immunoblotting

The Bad

It requires more than 30 micrograms of lysates per blot to detect it well.

The Bottom Line

This antibody works well for detecting the Cyclin A protein (~50 kD) in U2OS cell lysates via immunoblotting.

Share your experience with other scientists. Write a Review! »

Join the discussion