Biolegend
anti-CD3 BV421
317343
UCHT1
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This antibody was used to stain for T cells in human peripheral blood. It was chosen because it is a commonly used clone for CD3, BV421 is bright and works well with other fluorophores in the staining panel, and Biolegend has a good reputation for making quality flow cytometry antibodies.
Flow Cytometry
Whole blood
5 ul per test in 100uL staining volume for 15 minutes at room temperature
FcBlock + 0.2% BSA
N/A
LSR II
CD3 staining was excellent. Positive cells were fully resolved from negative.
Works well. Reproducible results.
Will continue to use this antibody.