Invitrogen
Anti-CLDN5 Antibody for Immunofluorescence
35-2500
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Decent antibody to label the tight junction Claudin-5.
Immunofluorescence
Adult mouse spinal cord
1:100, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS
2%Normal donkey serum in 0,3% triton-x100 in PBS
2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS
None.
Confocal Microscope.
Staining of tight junction Claudin-5. To obtain a good staining it is necessary to do an antigene retrieval. In this case, we boil the samples in the microwave during 5 minutes (with small breaks in between).
Quality.
Requires optimization.
Overall, this antibody is a good choice.