High Quality Total RNA Isolation Kit

University of Tennessee HSC
College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MACHEREY-NAGEL

Product Name:

NucleoSpin miRNA, Mini kit for miRNA and RNA purification

Catalog Number:

740971.250

This kits give you ease to isolate high quality total RNA from tissues or isolated cells. Just follow the manufacturer's protocol to get the best results. I have used this kit to isolate the total RNA from human mesenchymal stem cells for downstream gene expression analysis during different stress conditions. High quantity, as well as high quality total RNA, was obtained within 60 minutes of the sample processing.

Experimental Design and Results Summary

Application

Gene Expression

Starting Material

Human Mesenchymal stem cells

Tips

Perform cell lysis immediately at the end of the assay.

Results Summary

Total RNA with a good quantity and high quality was obtained within 60 minutes of the sample processing.

DOI or PMID #

N/A

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Summary

The Good

Easy and time saving kit. Also, we get protein pellet in between which can be used for western blot assays.

The Bad

Wash buffer is limited in the kit.

The Bottom Line

Think to use this kit for total RNA isolation for your samples,

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