Dependable Anti-human CD3 Antibody

College of Medicine
University of Tennessee HSC
Postdoctoral Researcher
December 21, 2021

College of Medicine
University of Tennessee HSC
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-human CD3 Antibody

Catalog Number:

317330

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: CD3 Antibodies

To stain CD3 population in human PBMCs, I have included this antibody in multi-color immuno-phenotyping flow panel. BV785 fluorochrome conjugated antibody was excited by violet laser resulted in clear distinct cloud positive for BV786, confirming CD3 population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:100

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

A clear distinct cloud positive for BV786 was observed confirming the CD3 positive cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works at a concentrations lower than that mentioned in the data sheet.

The Bad

Bit expensive.

The Bottom Line

Good choice for the multi-color flow panel.

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