Detects TRAP1 Well

R&D
EydisBio Inc.
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

TRAP1/HSP75 (D3D7N) Rabbit mAb

Catalog Number:

92345

Clone Number:

N/A

Info: Supplier Page 

Browse Similar Products: TRAP1 Antibodies

This antibody detected TRAP1 protein in western blot from human T cells. The bands were fuzzy but at the correct molecular weight. Further optimization of blocking reagent and incubation time and temperature may improve bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human T cells

Primary Incubation

1:500

Blocking Agent

NDS

Secondary Incubation

1:2000

Tertiary Incubation

N/A

Detection

Chemilumescence

Results Summary

This antibody needs some optimization for ideal bands if publication is the goal. There was some fuzziness to the bands during some incubations. The band was at the correct molecular weight.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Detects TRAP1

The Bad

Some background noise

The Bottom Line

Works well following optimization of blocking and incubations.

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