Reliable Mouse CD8 PerCP-Cy5.5

Surgery
UCSF
Graduate Student
November 30, 2021

BlueskyReddit
Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PerCP/Cyanine5.5 anti-mouse CD8a Antibody

Catalog Number:

100733

Clone Number:

N/A

Info: Supplier Page View Product Specs

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We used this antibody for out study of thymocyte development in the context of various inhibitors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymus

Primary Incubation

1:200 dilution for 20 minutes at room temp

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

This antibody shows very clean staining with sharp separation of CD8+ cells. Most of the thymocytes should be double positive and most are CD8+.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Stains great at low concentrations

The Bad

Is a tandem dye so spillover or degradation is a small concern

The Bottom Line

Overall a great antibody, works perfect for staining CD8

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