Works Ok, But Doesn't Have Clear Separation

Surgery
UCSF
Graduate Student
November 30, 2021

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse Ly-51 Antibody

Catalog Number:

108308

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: LY-51 Antibodies

We have been looking at perturbations to thymic cell composition and used this antibody to look at Ly51+ epithelial cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymus

Primary Incubation

1:200 dilution for 20 minutes at room temp

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

There is some staining, but the combination of relative rarity of Ly51+ cells in the thymus combined with poor separation makes it difficult to interpret overall.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Relatively cheap

The Bad

Poor separation

The Bottom Line

Gets the job done and is cheap, but if there's a cleaner alternative I'd recommend that

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