Dependable Donkey Anti-Rabbit IgG

Cardiovascular Biology
OMRF
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Jackson Immuno

Product Name:

Alexa Fluor® 647

Catalog Number:

711-607-003

Clone Number:

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-IgG Antibodies

Alexa Fluor 647 is a fluorescent compound with an excitation peak at 650 nm and an emission peak at 671 nm. It can be excited using a 640 nm laser paired with a 660/10 nm bandpass filter, a configuration that can be found.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse tissue liver

Primary Incubation

1:250 dilution overnight incubation

Blocking Agent

1% BSA prepared in 1X TBST

Secondary Incubation

1:250 dilution 1% BSA prepared in 1X TBST for 2hrs

Tertiary Incubation

None

Detection

Florescent microscope

Results Summary

Fab fragments are the antibody binding regions of an antibody. An IgG immunoglobulin contains two, comprising the arms of the classic Y shape. The Fab fragments can be isolated from the Fc portion, and from each other, by papain digestion. This results in a monovalent (one binding site) antibody.

DOI or PMID #

NA

Additional Notes

NA

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Summary

The Good

Great

The Bad

None

The Bottom Line

Very easy to use.

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