Dependable MitoTracker Dye

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Cardiovascular Biology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

MitoTracker™ Deep Red

Catalog Number:

M22426

Mito tracker red is a far red-fluorescent dye that stains mitochondria in live cells and can be used for mitochondrial localization. The dye is well-retained after aldehyde fixation and even subsequent permeabilization with detergents.

Experimental Design and Results Summary

Application

Staining

Starting Material

cell culture samples

Tips

High-sensitivity.

Results Summary

To label mitochondria, cells are simply incubated with MitoTracker probes, which passivelydiffuse across the plasma membrane and accumulate in active mitochondria. Once theirmitochondria are labeled, the cells can be treated with an aldehyde-based fixative for samplesthat need fixation to allow further processing of the sample.

DOI or PMID #

NA

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Summary

The Good

Great

The Bad

None

The Bottom Line

Mitochondrial staining works great.

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