Works Well for IHC

R&D
EydisBio Inc.
Principal Investigator
December 01, 2021

BlueskyReddit
R&D
EydisBio Inc.
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

HSP90 (C45G5) Rabbit mAb

Catalog Number:

4877

Clone Number:

This antibody worked well for western blot and IHC studies. Needed some optimization in IHC application due to the high expression level of Hsp90 in cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

DRG Nociceptors

Primary Incubation

1:1000

Blocking Agent

NDS

Secondary Incubation

1:2000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

This antibody worked well to detect Hsp90 isolated from DRG neurons from mice.

DOI or PMID #

Additional Notes

Related Categories

Image Gallery

Summary

The Good

WesternBlot application worked well

The Bad

High expression of target drug leads to optimization.

The Bottom Line

This antibody works well for western blot and IHC. The high expression of Hsp90 in cells may need some optimization for applications.

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