Consistent Immunostaining Results

Cardiovascular Biology
OMRF
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Thermo Scientific

Product Name:

CD68 Monoclonal Antibody

Catalog Number:

14-0681-80

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: CD68 Antibodies

CD68 has been widely used as a pan-macrophage marker. CD68 is a glycosylated type I membrane protein that belongs to the lysosome-associated membrane protein family of molecules. CD68 is predominantly expressed in late endosomes and lysosomes of macrophages, but is also found on the surface of DCs and osteoclasts.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse tissue liver

Primary Incubation

1:250 dilution overnight incubation

Blocking Agent

1% BSA prepared in 1X TBST

Secondary Incubation

1:250 dilution 1% BSA prepared in 1X TBST for 2hrs

Tertiary Incubation

None

Detection

Florescent microscope

Results Summary

CD68 is mostly localized in the late endosomes and lysosomes with a smaller fraction circulating to the cell surface. this antibody results very good .This antibody is highly recommended for use in immunostaining.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Great

The Bad

None

The Bottom Line

This antibody is highly recommended for others to use immunostaining experiments.

Share your experience with other scientists. Write a Review! »

Join the discussion