BD Biosciences
BD OptiBuild™ BUV395 Mouse Anti-Human CD134
745696
L106
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Our research aims at understanding how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors. CD4 T cells are usually identified as CD3+ CD4+ cells. In this experiment, we used anti-OX40/CD134 BUV395 antibody to identify OX40+ CD4 T cells.
Flow Cytometry
PMA+ionomycin+Calcium-activated healthy donor's PBMCs
Surface staining with anti-OX40 BUV395 at 1/25 and anti-CD3, anti-CD4 etc... (20' at 4°C, in the dark).
None
Staining with L/D dye (20' at 4°C, in the dark).
Cells were fixed and permeabilized with a commercial kit to counterstain for cytokines.
UV laser on Symphony cytometer
OX40 can be seen on CD4+ CD3+ T cells but the signal is very weak on activated primary cells.
The antibody is specific.
The antibody gives very dim signal and a FMO control is essential to set the positive threshold.. It is expensive
Can be used, but not ideal