ThermoFisher Scientific
Lipofectamine™ 3000 Transfection Reagent
L3000-015
Info: Supplier Page
Lipofectamine 3000 is a high efficiency, low cytotoxicity transfection reagent and I use it most of the time for transfection experiments. It provides reproducible results.
Cell culture
Human Mesenchymal Stem cells
Follow the steps/procedure mentioned with the reagent.
I observed more than 80% silencing of the target gene.
Low cytotoxicity
Costly
Recommends to use for the knock-down and knock-in assays.