Glycerol-Based Antifade Mountant for Confocal Imaging

University of Tennessee HSC
College of Medicine
Postdoctoral Researcher

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Company:

ThermoScientific Fisher

Product Name:

ProLong™ Diamond Antifade Mountant

Catalog Number:

P36961

For the mounting of the tissue samples on microscope slides stained with fluorophores, I have used this glycerol-based ProLong Diamond Antifade Mountant. Applied directly to the fluorescently labeled tissue samples, covered with the coverslip, and dried it. Mountant tissue sections were imaged with a confocal microscope after a month without any decrease in the intensity of the fluorophores.

Experimental Design and Results Summary

Application

Confocal Imaging

Starting Material

Mice tissue sections

Tips

Just add a drop of the mountant.

Results Summary

ProLong Diamond Antifade mountant tissue sections were imaged with a confocal microscope and showed bright fluorophores signals.

DOI or PMID #

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Summary

The Good

Provide long time window to image the samples in fluorescence microscope.

The Bad

Bit expensive

The Bottom Line

ProLong Diamond Antifade mountant is a good choice to protect fluorescent dyes and fluorescent protein from fading in your target samples.

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