Suitable FBS-Containing Buffer for Staining Flow Cells

University of Tennessee HSC
College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

Stain Buffer (FBS)

Catalog Number:

554656

I regularly use this BD Biosciences stain buffer for staining single cell suspension at different staining steps prepared for flow cytometry acquisition.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Human PBMCs

Tips

Always use chilled buffer

Results Summary

I observed cells were viable throughout the process of staining and acquired 90% live cells for most of the samples.

DOI or PMID #

Related Categories

Image Gallery

Summary

The Good

Suitable for any type of cells surface marker staining for flow.

The Bad

None

The Bottom Line

Cells are happy in this buffer and decrease the chances of cell death, which makes this buffer a good one to stain your cells for flow cytometry.

Join the discussion