Easy to Detect Phospho SAPK/JNK from CST

IMCB
NTHU
Graduate Student
November 19, 2021

BlueskyReddit
IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technology

Product Name:

Phospho-SAPK/JNK (Thr183/Tyr185)

Catalog Number:

4668

Clone Number:

N/A

It detects endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with SAPK/JNK singly phosphorylated at Tyr185. This antibody may cross-react with phosphorylated p44/42 or p38 MAP kinases.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Immune Cell

Primary Incubation

1:1000, Overnight, 4deg

Blocking Agent

3% BSA

Secondary Incubation

1:10000, 1hr, RT

Tertiary Incubation

NIL

Detection

Chemiluminescence

Results Summary

There are two clear bands seen from the result and it coincides with the data sheet that was provided by the company.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use and optimise

The Bad

Might cross react with phosphorylated p44/42 or p38 MAP kinases.

The Bottom Line

Easy to optimize and detect, it is a antibody that is highly recommended to those who needs to determine the level of phosphorylation in SAPK/JNK.

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