Thermo Fisher Scientific
Pierce™ BCA Protein Assay Kit
23227
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I regularly use this protein assay kit for most of the experiments for western blotting, protein normalization across the assay, and proteomics samples. Have a good linear range for protein estimation in the test samples from 20 µg/mL to 2000 µg/mL. The kit also contains the stock vials of 2 mg/ml BSA.
Protein estimation
Cell lysates, tissue lysates and cell supernatant
Dilute the stock BSA for the standard curve in the same buffer in which you have your test samples.
Gradient purple color observed in the BSA standard samples and differential in test samples showing different protein concentrations in the test samples. After taking absorbance at 562 nm in the spectrophotometer, I observed a linear BSA standard curve with r2=0.997 and a good concentration of proteins in the test samples.
BSA stock 2 mg/ml is given with the kit.
Not compatible for samples with peptides with less than 2000 Da.
Excellent product to use for the protein estimation for your daily research related to proteins.