Mouse MHCII AF700 Works Well

Surgery
UCSF
Graduate Student
November 18, 2021

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Alexa Fluor® 700 anti-mouse I-A/I-E Antibody

Catalog Number:

107621

Clone Number:

N/A

Info: Supplier Page View Product Specs

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We were looking in mouse lymph nodes at antigen presenting cells, and used MHCII staining to identify these cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:200 dilution for 30 minutes at 4 degrees C

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

Used this antibody along with CD11c to identify dendritic cells in the mouse lymph node.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Stains great at low concentrations

The Bad

Has a little bleedover into nearby channels

The Bottom Line

Overall a great antibody for mouse immune cells, will use again

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