Mouse CD11c PE-Cy7 Gives Great Results

Surgery
UCSF
Graduate Student
November 22, 2021

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cyanine7 anti-mouse CD11c Antibody

Catalog Number:

117317

Clone Number:

Analyzing mouse lymph nodes for antigen presenting cells, specifically CD11c+ dendritic cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:200 dilution for 30 minutes at 4 degrees C

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

This product enabled the identification of CD11c+ dendritic cells and their further characterization as antigen presenting cells.

DOI or PMID #

Additional Notes

Related Categories

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Summary

The Good

Stains great at low concentrations

The Bad

Tandem dye, so not the most stable once fixed

The Bottom Line

Great antibody as long as you run FACs within a few days, will use again

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