Biolegend
PerCP anti-mouse CD45 Antibody
103129
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Studying antigen presenting cells in lymph nodes, using CD45 to identify immune cells.
Flow Cytometry
Mouse lymph nodes
1:200 dilution for 30 minutes at 4 degrees C
2.4G2 Fc Block
N/A
LSRII FACS Analyzer
Used CD45 to identify immune cells and eliminate non-hemopoietic cells from analysis.
Stains well at low concentration
PerCP isn't the best fluorochrome
Great consistent staining, would use again.