Reliable Mouse CD45 PerCP

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PerCP anti-mouse CD45 Antibody

Catalog Number:

103129

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: CD45 Antibodies

Studying antigen presenting cells in lymph nodes, using CD45 to identify immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:200 dilution for 30 minutes at 4 degrees C

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

Used CD45 to identify immune cells and eliminate non-hemopoietic cells from analysis.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Stains well at low concentration

The Bad

PerCP isn't the best fluorochrome

The Bottom Line

Great consistent staining, would use again.

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