Mouse BV650 CD44 Does The Job Well

Surgery
UCSF
Graduate Student
November 22, 2021

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 650™ anti-mouse/human CD44 Antibody

Catalog Number:

103049

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: CD44 Antibodies

Stained splenocytes to look at breakdown of memory/naive T cell subsets using CD44 and CD62L antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 dilution for 30 minutes at 4 degrees C

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

This antibody helped me confirm that the mouse spleen contains many more naive T cells than memory T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Clean signal

The Bad

A little expensive

The Bottom Line

A good choice for identifying memory T cells

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