Mouse CD3 BUV395 Works Great

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV395 Mouse Anti-Human CD3

Catalog Number:

563548

Clone Number:

N/A

Info: Supplier Page

Browse Similar Products: CD3 Antibodies

I used this antibody in a mouse splenocyte panel to identify T cells. It works well, has minimal bleedover and shows clean separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200 dilution for 30 minutes at 4 degrees C

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

Allowed easy and clean identification of CD3+ T cells in the spleen.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very clean separation for identifying T cells

The Bad

A bit expensive compared to other CD3 antibodies

The Bottom Line

Overall a great antibody, just a little pricy

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