Excellent Antibody to Detect LMCD1 in Human Cell Line

Physiology
University of Tennessee HSC
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

LMCD1 Antibody

Catalog Number:

ab121788

Clone Number:

Info:Supplier Page View Product Specs

Browse Similar Products: LMCD1 Antibodies

The aim of the study was to test the role of specific treatment on LMCD1 protein levels in the human primary cell line. Tissue proteins from each group of mice treatment were extracted and resolved in SDS PAGE. Resolved proteins were western transferred in the nitrocellulose membrane and immunoprobed for LMCD1 antibody obtained from Abcam.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human primary cell extract

Primary Incubation

Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius

Blocking Agent

5% skimmed milk made in 1x TBST

Secondary Incubation

Goat anti-rabbit IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in1x TBST, 2hr at RT

Tertiary Incubation

None

Detection

Enhanced chemiluminescent detection in X-Ray film

Results Summary

The excellent and bright band of LMCD1 was observed. No nonspecific band was observed with this antibody.

DOI or PMID #

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

It gives a specific band of LMCD1

The Bad

None

The Bottom Line

Excellent antibody

Share your experience with other scientists. Write a Review! »

Join the discussion