MyBioSource.com
STAT1 antibody
MBS851202
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To normalize the immunoblotted nitrocellulose membrane for the pSTAT1 levels, STAT1 levels were detected in the same blot. For, this the immunoblots which were already identified for a pSTAT1 was IgG stripped with stripping buffer, blocked with skim milk, and probed with MyBioSource.com STAT1 antibody. The selection of antibody was based on previous experience and the antibody flyer.
Western Blot
Human cell extract
Dilution (1:1000) in 5% BSA prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in 1x TBST
Goat anti-Rabbit-IgG HRP Antibody, dilution (1:4000) in 5% skimmed milk prepared in 1x TBST, 2hr at RT
None
ECL detection in X-Ray film
The antibody has detected a very strong nonspecific band at nearly 50 kDa. A thin band of STAT1 was also detected by this antibody.
Faint STAT1 protein was detected.
A dark nonspecific band was detected
Average antibody