Easy to Detect HIF1a Antibody

IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Tech

Product Name:

HIF-1α Rabbit mAb

Catalog Number:

36169

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: HIF1a Antibodies

HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. This antibody is used to detect the protein expression of HIF1a.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine Immune Cell

Primary Incubation

1:1000 3% BSA O/N 4deg

Blocking Agent

3% BSA

Secondary Incubation

1:10000 3% BSA RT 1hr

Tertiary Incubation

NIL

Detection

Chemiluminescence

Results Summary

A single band is obtained and there is no non specific bands detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use and highly specific

The Bad

NIL

The Bottom Line

A highly recommended antibody for those who seek to use it to detect the expression of HIF1a protein in cells.

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