This CD9 Antibody Works Well

medicine
Brigham and Women's Hospital and Harvard Medical School
Research Scientist
October 29, 2021

BlueskyReddit
medicine
Brigham and Women's Hospital and Harvard Medical School
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

CD9 antibody

Catalog Number:

20597-1-AP

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: CD9 Antibodies

My research field is infectious disease. We investigate the extracellular vesicles. We use this antibody as a marker to validate the purified EVs.

Experimental Design and Results Summary

Applications

Western Blot

Sample

exosomes purified from Raw264.7 and differentiated THP-1 cells

Primary Incubation

1:500 dilution for over night at 4C

Blocking Agent

1% BSA in PBS

Secondary Incubation

1:5000 for 1 hour at room temperature

Tertiary Incubation

N/A

Detection

HRP chemiluminescence

Results Summary

This antibody works for detection of CD9 from EVs purified from mammalian EVs. However, it seems that a large amount (50 ug EVs) of EVs is needed to get the signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good signal to detect CD9 from large amount of EVs

The Bad

You'll need large amount of EVs

The Bottom Line

I recommend it

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