Reliable Secondary Antibody for Confocal Microscopy

Research & Development
Covaris
Research Scientist
November 01, 2021

BlueskyReddit
Research & Development
Covaris
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 555

Catalog Number:

A28180

Clone Number:

N/A

The red dots are the images from the confocal microscope showing the binding of the antibody with it's primary target

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human T cells

Primary Incubation

1:1000

Blocking Agent

5% Milk

Secondary Incubation

1:1000

Tertiary Incubation

N/A

Detection

Confocal microscope

Results Summary

The increase in red dots was observed during a chemical treatment

DOI or PMID #

https://doi.org/10.1186/s12979-019-0153-z

Additional Notes

N/A

Image Gallery

Summary

The Good

Very easy to detect

The Bad

N/A

The Bottom Line

Great antibody for confocal microscopy

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