Consistent 53BP1 Antibody

Department of Internal Medicine
East Tennessee State University
Graduate Student
October 29, 2021

Department of Internal Medicine
East Tennessee State University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

53BP1 Antibody

Catalog Number:

4937S

Clone Number:

N/A

Info: Supplier Page View Product Specs

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The anti-rabbit 53BP1 antibody was used to perform confocal microscopy. The secondary antibody used here was anti-rabbit IgG-Alexa Fluor 488.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human T cells

Primary Incubation

1:1000

Blocking Agent

5% Milk

Secondary Incubation

anti-rabbit IgG-Alexa Fluor 488 (1:1000)

Tertiary Incubation

N/A

Detection

Confocal microscope

Results Summary

The anti-rabbit antibody was localized in the DNA damage sites as shown in green fluorescence.

DOI or PMID #

https://doi.org/10.1186/s12979-019-0153-z

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very easy to detect

The Bad

N/A

The Bottom Line

The secondary antibody shall be incubated for slightly longer hours (3-4 hrs at RT)

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