Synaptic systems
cFOS antibody
226004
Info: Supplier Page
Actively firing neurons were stained with cFOS staining. however the staining was not efficient and most of the dapi positive cells were labeled with cFOS antibody in the whole cortex.
Immunofluorescence
Postnatal mouse brain tissue-P12, cryosections
1:300, 4° C overnight
10% NDS in PBST (0.5% TritonX 100)
1:500, AF 488
none
not efficient labelling,
Works better with Antigen retrieval (10mM Sodium citrate-boiling)
Labelling could be good if standardized
High background, needs to be used with another efficient cFOS antibody
Not recommended, requires a lot of standardization