Cell Signaling
cFOS antibody
2250
9F6
Info: Supplier Page
cFOS antibody was used to determine the actively firing neurons in the postnatal cortex at P12. Labelling is overall good. layer II-III shows many cFOS positive cells.
Immunofluorescence
Postnatal mouse brain tissue-P12, cryosections
1:300, 4° C overnight
10% NDS in PBST (0.5% TritonX 100)
1:500, AF568
none
determines the actively firing neurons in the postnatal cortex
Works better with Antigen retrieval (10mM Sodium citrate-boiling)
Specific staining
High background
Recommended. however standardization is important. would buy again