Easy to Use Kit for Detecting EdU+ Cells

Emory University
Microbiology & Immunology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Click-It Plus EdU Alexa Fluor 647 Flow Cytometry Kit

Catalog Number:

C10635

Info: Supplier Page 

We used this kit to detect EdU+ T cells in the lungs of mice following infection. This kit was easy to optimize for our needs and resulted in clear staining

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Murine lung cells

Tips

Protocol was followed exactly. Samples were analyzed on a cytometer the following morning

Results Summary

We were able to reliably identify EdU+ CD8+ T cells with minimal background from the lungs of mice following infection.

DOI or PMID #

N/A

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Summary

The Good

Easy to use, good separation of positive and negative populations

The Bad

None

The Bottom Line

Kit is easy to use and staining is reliable. Would buy again

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