Unspecific Bands at Wrong Sizes

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

anti-PDK4 antibody

Catalog Number:

PA5-13776

Clone Number:

N/A

Info: Supplier Page 

Investigating insulin-dependent and insulin-independent signaling pathways under the context of obesity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

mouse primary t cells

Primary Incubation

1:1000

Blocking Agent

5% non milk TBST

Secondary Incubation

1:10,000 in 5% milk TBST dor 1hr

Tertiary Incubation

N/A

Detection

HRP

Results Summary

Only unspecific bands at wrong sizes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

N/A

The Bad

No signal. Unspecific bands

The Bottom Line

I would not recommend this antibody for western blot.

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