Biolegend
PE/Dazzle™ 594 anti-human CD4 Antibody
300548
RPA-T4
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Our research aims at understanding how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors. CD4 T cells are usually identified as CD3+ CD4+ cells. In this experiment, we used anti CD4 antibodies to identify CD4 T cells
Flow Cytometry
PMA/ionomycin/Calcium-stimulated and unstimulated healthy donor's PBMCs
Surface staining with anti-CD4 PE Dazzle594 1/200 (20' at 4°C, in the dark)
None
Staining with L/D dye (20' at 4°C, in the dark).
Cells were fixed and permeabilized with a commercial kit to counterstain for cytokines.
Yellow-green laser on Symphony cytometer
CD4+ T cells could be detected even after stimulation.
Bright even after stimulation of the cells
Can spread to other channels
Very good option.