BD Pharmingen
PerCP-Cy™5.5 Mouse Anti-Human HLA-DR
560652
G46-6 (RUO)
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Our research aims at understanding how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors. CD4 T cells are usually identified as CD3+ CD4+ cells. In this experiment, we used anti HLA-DR antibodies to exclude non-T cells.
Flow Cytometry
Unstimulated healthy donor's PBMCs
Surface staining with anti- HLA-DR Percp-Cy5.5 1/50 (20' at 4°C, in the dark).
none
Staining with L/D dye (20' at 4°C, in the dark).
Cells were fixed and permeabilized with a commercial kit to counterstain for cytokines.
Blue laser on Symphony cytometer
We could identify HLA-DR+CD3- live cells.
Bright staining, easy to set
N/A
Suitable.