Ab8227 from Abcam Is Dependable

September 30, 2021

BlueskyReddit
Biology
Stanford University
Graduate Student

Overall

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Company:

Abcam

Product Name:

Anti-beta Actin antibody

Catalog Number:

ab8227

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-Beta Antibodies

This is high titer great antibody to detect b-actin as loading control for mouse liver lysates.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse liver lysates

Primary Incubation

1:2000 overnight in cold room

Blocking Agent

Odyssey blocking buffer

Secondary Incubation

goat at rabbit:780

Tertiary Incubation

1:10000 room temperature for 1hr

Detection

LiCOR

Results Summary

This is western blot on mouse liver lysates using rabbit anti-b-actin antibody. This antibody recongnizes two bands consistently.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It's recognizing the predicted band and has high titer

The Bad

it recongnizes two bands somehow

The Bottom Line

this is a good antibody detecting b-actin as loading control

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