Sensitive Anti-Beta-Actin Antibody for Western Blot

September 10, 2021

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Mouse monoclonal anti- beta Actin [AC-15] (HRP)

Catalog Number:

AB49900

Clone Number:

N/A

Investigating insulin-dependent and insulin-independent signaling pathways under the context of obesity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human SGBS cells

Primary Incubation

1:10000 30 min at RT in TBST buffer

Blocking Agent

4%BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

HRP substrate and x-ray film exposure

Results Summary

Good signal across all samples. Used as a loading control.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good signal

The Bad

Expensive

The Bottom Line

Antibody has great signal, highly recommended.

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