Highly Sensitive Substrate for Western Blot

PennState College of Medicine
Pharmacology
Postdoctoral Researcher

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Company:

ThermoScientific

Product Name:

SuperSignal West Pico Plus Chemiluminescent substrate

Catalog Number:

34580

SuperSignal West Pico chemiluminescent substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate to detect proteins in picogram to femtogram-level. The substrate is highly sensitive. Very good product from Thermo.

Experimental Design and Results Summary

Application

Western Blot

Starting Material

N/A

Tips

Cell lysate or purified proteins

Results Summary

Stable and highly sensitive. After adding the substrate, two minutes of incubation is recommended. Detects even low amount of proteins. Pico plus has better sensitivity compared to other Supersignal substrates from Thermo.

DOI or PMID #

N/A

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Summary

The Good

Sensitivity and ease of use.

The Bad

None

The Bottom Line

Excellent ECL substrate for western blot. Highly recommended.

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