Reliable IFN Antibody

September 09, 2021

BlueskyReddit
BMS
UCSF
Graduate Student

Overall

Quality of Results

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Company:

BD

Product Name:

APC Mouse Anti-Human IFN-γ

Catalog Number:

554702

Clone Number:

N/A

Info: Supplier Page 

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Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:50 in Perm buffer at RT for 60 min

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

A strong IFNg signal was detected in CD8+ T cells from human PBMCs.

DOI or PMID #

N/A

Additional Notes

4% PFA fixation followed by 1x perm buffer (eBioscience) permeabilization was performed prior to intracellular staining.

Image Gallery

Summary

The Good

Reliable and good signal.

The Bad

Expensive.

The Bottom Line

Antibody has great signal, highly recommended.

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