Hard to Optimize IL-22

September 03, 2021

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen

Product Name:

APC IL-22 Monoclonal Antibody

Catalog Number:

17-7222-82

Clone Number:

N/A

I've tried many times with this antibody but never got a good staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse ear skin tissue

Primary Incubation

1:100 in Perm Buffer at RT for 45 min to 1 hr

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Tried my ICS staining protocol but failed to get a good positive population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

NA

The Bad

difficult to optimize to get a good staining

The Bottom Line

Not recommended for in vivo cell isolation and staining. It might work better for in vitro Th17 cell differentiation staining.

Share your experience with other scientists. Write a Review! »

Join the discussion