Invitrogen
eBioscience™ Fixable Viability Dye eFluor™ 780
65-0865-14
NA
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Good viability dye to get rid of dead cells. This is also compatible with PFA-based fixation and permeabilization for downstream intracellular staining.
Flow Cytometry
mouse draining lymph node
1:100) in FACS Buffer at RT for 30 min
great resolution from negative vs. positive populations.
consistent results across different batches
pricy without academic discount
Make sure have a good single color control for flow. I used 70% ethanol to kill my cells and then stain with the fixable viability dye.