Great CD3 Antibody for Flow

August 27, 2021

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse CD3ε Antibody

Catalog Number:

100305

Clone Number:

145-2C11

Reliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse ear skin tissue

Primary Incubation

1:100 in FACS Buffer at RT for 30 min

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Great resolution from negative vs. positive populations.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

consistent results across different batches

The Bad

N/A

The Bottom Line

This clone is the one I stick to for mouse CD3+ staining.

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