Reliable CD8a Antibody for Flow Cytometry

August 27, 2021

BlueskyReddit
BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 605™ anti-mouse CD8a

Catalog Number:

100744

Clone Number:

53-6.7

Reliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse draining lymph node

Primary Incubation

1:100 in FACS Buffer at RT for 30 min

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Good resolution from negative vs. positve population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Consistent results across different batches.

The Bad

NA

The Bottom Line

Reliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.

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