Amazing CD45 Antibody

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 785™ anti-mouse CD45.2 Antibody

Catalog Number:

109839

Clone Number:

104

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse ear skin tissue

Primary Incubation

30min

Blocking Agent

N/A

Secondary Incubation

1:100

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Good negative and positive sepAration from mouse ear tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

consistent results across different batches

The Bad

N/A

The Bottom Line

try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.

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