Reliable GFP Antibody for IHC-P

PRIMATE RESEARCH CENTER
UNIVERSITY OF WISCONSIN
Scientist I

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

GFP antibody

Catalog Number:

NB100-1770

Clone Number:

Polyclonal

This antibody was used to detect GFP postive human iPSC cells in a paraffin section of a teratoma. The antibody was easyt o optimize and did not give any background.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human iPSC teratoma section

Primary Incubation

1:500 Overnight at 4oC

Blocking Agent

5% serum

Secondary Incubation

1:500, 1h at RT

Tertiary Incubation

None

Detection

ABC detection

Results Summary

This antibody is very helpful to detect GFP positive cells. There was no background seen and it was easy to optimize.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

no unwanted background

The Bad

None

The Bottom Line

very good antibody to use for detecting GFP positive cells in IHC-P. The anitbody does not give unwanted background staining.

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