GFP Antibody for IHC-P

PRIMATE RESEARCH CENTER
UNIVERSITY OF WISCONSIN
Scientist I

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

GFP antibody

Catalog Number:

NB600-303

Clone Number:

Polyclonal

This antibody was used to detected GFP positive human iPSC cells in a teratoma section. The antibody was easy to use and effectively detected GFP positive cells in the paraffin section.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human iPSC teratoma section

Primary Incubation

1:500 Overnight at 4oC

Blocking Agent

5% serum

Secondary Incubation

1:1000, 1h at RT

Tertiary Incubation

none

Detection

ABC detection

Results Summary

This antibody was very effective in detecting GFP positive cells in the teratoma sections. It developed efficient signal at the dilution used.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

A good antibody to use for GFP positive cells

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