Biolegend
PE/Dazzle™ 594 anti-mouse CD38 Antibody
102730
90
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) in health and disease. Here, we aimed at titrating anti-CD38 to use in future experiments.
Flow Cytometry
mouse spleen WT
Surface staining with CD38 PE-Dazzle594 1/800, 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
As it can be observed in the figure, a dilution of 1/800 gives a good separation of positive and negative cells.
Accessible and high quality
Antibody has great signal, highly recommended.