CCR7 Antibody Works Great

September 30, 2021

BlueskyReddit
Diabetes Center
UCSF
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

CCR7-Biotin Antibody

Catalog Number:

120104

Clone Number:

4B12

Our lab is interested in mechanisms of peripheral and central immune tolerance as it pertains to tumor and transplant immunology.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node, spleen

Primary Incubation

1:50 dilution, stain at 37C for 30 minutes

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This biotin conjugated CCR7 antibody binds well to CCR7 in mouse samples, and we have used it in conjunction with multiple SA-fluorophore combinations in our experiments with good staining and population separation. We use this antibody primarily for distinction between DC subsets.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Binds well, useful with multiple different fluorophores since biotin conjugated

The Bad

Has to be incubated at 37C for optimal binding, which may affect cell viability

The Bottom Line

This CCR7 antibody works well for distinction between DC subsets.

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