MHCII I-AB eF450

August 20, 2021

BlueskyReddit
Diabetes Center
UCSF
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Life Technologies

Product Name:

Anti-Mouse MHCII I-AB eF450

Catalog Number:

48-5320-82

Clone Number:

N/A

Our lab is interested in mechanisms of peripheral and central immune tolerance as it pertains to tumor and transplant immunology.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Lymph Node, Spleen, Thymus

Primary Incubation

1:200 Dilution, 30 minutes on ice

Blocking Agent

N/a

Secondary Incubation

N/a

Tertiary Incubation

N/a

Detection

Flow cytometry

Results Summary

This product stains well for MHCII I-AB and produces a narrow peak with good separation of MHCII positive and negative cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Low dilution concentration so antibody lasts a long time, sharp population distinction

The Bad

Only I-AB subsets of MHCII

The Bottom Line

This antibody works well for staining of MHCII positive immune cell subsets in peripheral populations (lymph nodes, spleen) and in the thymus.

Share your experience with other scientists. Write a Review! »

Join the discussion