Great Antibody for Flow Staining

August 20, 2021

BlueskyReddit
Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse CCR7 (CD197) Biotin

Catalog Number:

120104

Clone Number:

4B12

We use this antibody regularly to stain migratory dendritic cells from the lymph nodes. It requires special 37C incubation for 30min, but otherwise works great.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:50, 30 min, 37C

Blocking Agent

1% BSA

Secondary Incubation

Streptavidin BV785, 1:100, 30min 4C

Tertiary Incubation

none

Detection

Flow cytometry

Results Summary

We use this antibody regularly to stain migratory dendritic cells from the lymph nodes. It requires special 37C incubation for 30min, but otherwise works great.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

none

The Bottom Line

Antibody has great signal when you follow the right staining condition, highly recommended.

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